Journal: Antimicrobial Agents and Chemotherapy

Article Title: Differential In Vitro Activities of Individual Drugs and Bedaquiline-Rifabutin Combinations against Actively Multiplying and Nutrient-Starved Mycobacterium abscessus

doi: 10.1128/AAC.02179-20

Figure Lengend Snippet: In vitro drug activity against actively growing and nutrient-starved M. abscessus. Bacterial populations were exposed to amikacin (A), bedaquiline (B), clofazimine (C), imipenem (D), linezolid (E), and rifabutin (F) for up to 7 days in each of the following conditions: cation-adjusted Mueller-Hinton broth (CAMHB) (left panel); nutrient starvation in PBS for 7 days prior to drug exposure (NS-7, middle panel); and nutrient starvation in PBS for 14 days prior to drug exposure (NS-14, right panel). Overgrowth and clumping of bacteria in CAMHB precluded CFU determination in all no-drug controls and in some of the samples with lower drug concentrations at day 3 and/or day 7. The lower limit of detection was 0.48 log10 CFU/ml. All CFU data are provided in Tables S1 to S6 in the supplemental material.

Article Snippet: MIC/MBC of bedaquiline and rifabutin alone for M. abscessus in CAMHB and Middlebrook 7H9 broth.

Techniques: In Vitro, Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Differential In Vitro Activities of Individual Drugs and Bedaquiline-Rifabutin Combinations against Actively Multiplying and Nutrient-Starved Mycobacterium abscessus

doi: 10.1128/AAC.02179-20

Figure Lengend Snippet: In vitro clarithromycin activity against actively growing and nutrient-starved M. abscessus. Bacterial populations were exposed to clarithromycin for up to 14 days in the following conditions: cation-adjusted Mueller-Hinton broth (CAMHB) (left panel); nutrient starvation for 7 days prior to drug exposure (middle panel); and nutrient starvation for 14 days prior to drug exposure (right panel). Overgrowth and clumping of bacteria precluded accurate CFU determination in the no-drug controls in CAMHB. The lower limit of detection was 0.48 log10 CFU/ml. All CFU data are provided in Table S7.

Article Snippet: MIC/MBC of bedaquiline and rifabutin alone for M. abscessus in CAMHB and Middlebrook 7H9 broth.

Techniques: In Vitro, Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Differential In Vitro Activities of Individual Drugs and Bedaquiline-Rifabutin Combinations against Actively Multiplying and Nutrient-Starved Mycobacterium abscessus

doi: 10.1128/AAC.02179-20

Figure Lengend Snippet: In vitro activity of bedaquiline (BDQ) alone and in combination with rifabutin (RFB) against actively growing and nutrient-starved M. abscessus. Bacterial populations were exposed to bedaquiline alone (A), or bedaquiline plus rifabutin at fixed concentrations of 1 μg/ml (B), 2 μg/ml (C), or 4 μg/ml (D) in the following conditions: cation-adjusted Mueller-Hinton broth (CAMHB, left panel); 7H9 broth with 10% Middlebrook oleic acid-albumin-dextrose-catalase (OADC) supplement (middle panel); and nutrient starvation in PBS for 14 days (NS-14) prior to drug exposure (right panel). Overgrowth and clumping of bacteria in CAMHB or 7H9 broth precluded CFU determination for the no-drug controls and some samples with lower drug concentrations at day 7. The lower limit of detection was 0.48 log10 CFU/ml. All CFU data are provided in Tables S2 and S9.

Article Snippet: MIC/MBC of bedaquiline and rifabutin alone for M. abscessus in CAMHB and Middlebrook 7H9 broth.

Techniques: In Vitro, Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Differential In Vitro Activities of Individual Drugs and Bedaquiline-Rifabutin Combinations against Actively Multiplying and Nutrient-Starved Mycobacterium abscessus

doi: 10.1128/AAC.02179-20

Figure Lengend Snippet: In vitro activity of rifabutin (RFB) alone and in combination with bedaquiline (BDQ) against actively growing and nutrient-starved M. abscessus. Bacterial populations were exposed to rifabutin alone (A), or rifabutin plus bedaquiline at fixed concentrations of 0.03125 μg/ml (B) or 0.125 μg/ml (C) in the following conditions: cation-adjusted Mueller-Hinton broth (CAMHB, left panel); 7H9 broth with 10% Middlebrook oleic acid-albumin-dextrose-catalase (OADC) supplement (middle panel); and nutrient starvation in PBS for 14 days (NS-14) prior to drug exposure (right panel). Overgrowth and clumping of bacteria in CAMHB or 7H9 broth precluded CFU determination for the no-drug controls and some samples with lower drug concentrations at day 3 or day 7. The lower limit of detection was 0.48 log10 CFU/ml. All CFU data are provided in Tables S6 and S10.

Article Snippet: MIC/MBC of bedaquiline and rifabutin alone for M. abscessus in CAMHB and Middlebrook 7H9 broth.

Techniques: In Vitro, Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Differential In Vitro Activities of Individual Drugs and Bedaquiline-Rifabutin Combinations against Actively Multiplying and Nutrient-Starved Mycobacterium abscessus

doi: 10.1128/AAC.02179-20

Figure Lengend Snippet: In vitro activity of bedaquiline (BDQ) alone and in combination with rifabutin (RFB) and amikacin (AMK) against actively growing and nutrient-starved M. abscessus. Bacterial populations were exposed to bedaquiline alone (A), or bedaquiline plus rifabutin at fixed concentrations of 1 μg/ml (B and D) or 2 μg/ml (C and E), and amikacin at fixed concentrations of 4 μg/ml (B and C) or 16 μg/ml (C and D), in the following conditions: cation-adjusted Mueller-Hinton broth (CAMHB, left panel) and nutrient starvation in PBS for 14 days (NS-14) prior to drug exposure (right panel). Overgrowth and clumping of bacteria in CAMHB precluded CFU determination for the no-drug controls and some samples with lower drug concentrations at day 7. The lower limit of detection was 0.48 log10 CFU/ml. All CFU data, including data for additional bedaquiline concentrations not included in the graphs, are provided in Tables S2 and S11.

Article Snippet: MIC/MBC of bedaquiline and rifabutin alone for M. abscessus in CAMHB and Middlebrook 7H9 broth.

Techniques: In Vitro, Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Synergistic Efficacy of β-Lactam Combinations against Mycobacterium abscessus Pulmonary Infection in Mice

doi: 10.1128/AAC.00614-19

Figure Lengend Snippet: Mean CFU in the lungs of mice infected with M. abscessus ATCC 19977 and treated with antibiotics across 4 weeks, with associated reduction in lung CFU compared to untreated controls

Article Snippet: All mice were infected concurrently with 10 ml M. abscessus culture at an A 600 of 0.1 via aerosolization using a Glas-Col inhalation exposure system (Glas-Col, Terre Haute, Indiana) according to the manufacturer’s instructions.

Techniques: Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Synergistic Efficacy of β-Lactam Combinations against Mycobacterium abscessus Pulmonary Infection in Mice

doi: 10.1128/AAC.00614-19

Figure Lengend Snippet: M. abscessus CFU in the lungs of mice treated with antibiotic combinations versus single antibiotics versus untreated controls. All mice were immunosuppressed with dexamethasone. Data points represent individual mice. (A) Imipenem and cefdinir; (B) imipenem and doripenem; (C) imipenem and biapenem; (D) biapenem and avibactam.

Article Snippet: All mice were infected concurrently with 10 ml M. abscessus culture at an A 600 of 0.1 via aerosolization using a Glas-Col inhalation exposure system (Glas-Col, Terre Haute, Indiana) according to the manufacturer’s instructions.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Synergistic Efficacy of β-Lactam Combinations against Mycobacterium abscessus Pulmonary Infection in Mice

doi: 10.1128/AAC.00614-19

Figure Lengend Snippet: Regression analysis of observed M. abscessus burden in the lungs of mice

Article Snippet: All mice were infected concurrently with 10 ml M. abscessus culture at an A 600 of 0.1 via aerosolization using a Glas-Col inhalation exposure system (Glas-Col, Terre Haute, Indiana) according to the manufacturer’s instructions.

Techniques:

Journal: Infection and Drug Resistance

Article Title: The Homologous Gene of Chromosomal Virulence D ( chvD ) Presents High Resolution as a Novel Biomarker in Mycobacterium Species Identification

doi: 10.2147/IDR.S422191

Figure Lengend Snippet: chvD sequence matches among types/reference strains

Article Snippet: M. abscessus subsp. bolletii , BD , AP018436.1 , RGM , M. abscessus subsp. massiliense , 96.88% , M. chelonae , 88.83%.

Techniques: Sequencing

Journal: Infection and Drug Resistance

Article Title: The Homologous Gene of Chromosomal Virulence D ( chvD ) Presents High Resolution as a Novel Biomarker in Mycobacterium Species Identification

doi: 10.2147/IDR.S422191

Figure Lengend Snippet: Comparison of mycobacteria identified by 16S rRNA/ITS/ hsp65 / rpoB gene sequencing and chvD sequencing among the 179 clinical isolates a

Article Snippet: M. abscessus subsp. bolletii , BD , AP018436.1 , RGM , M. abscessus subsp. massiliense , 96.88% , M. chelonae , 88.83%.

Techniques: Comparison, Sequencing

Journal: GigaByte

Article Title: Mycobacterial metabolic model development for drug target identification

doi: 10.46471/gigabyte.80

Figure Lengend Snippet: Mycobacterial metabolic model development for drug target identification: My. abscessus and M. leprae .

Article Snippet: Automated draft reconstructions of M. leprae and My. abscessus were downloaded from BioModels and evaluated against other organism-specific databases, such as BioCyc, (RRID: SCR_002298 ) and Kyoto Encyclopedia of Genes and Genomes (KEGG, RRID: SCR_012773 ) [ , ] .

Techniques: Drug discovery

Journal: GigaByte

Article Title: Mycobacterial metabolic model development for drug target identification

doi: 10.46471/gigabyte.80

Figure Lengend Snippet: Overall capability of the models: My. abscessus ( i Mab22) and M. leprae and ( i Mlep22).

Article Snippet: Automated draft reconstructions of M. leprae and My. abscessus were downloaded from BioModels and evaluated against other organism-specific databases, such as BioCyc, (RRID: SCR_002298 ) and Kyoto Encyclopedia of Genes and Genomes (KEGG, RRID: SCR_012773 ) [ , ] .

Techniques:

Journal: GigaByte

Article Title: Mycobacterial metabolic model development for drug target identification

doi: 10.46471/gigabyte.80

Figure Lengend Snippet: Unique/bottleneck reactions in My. abscessus and M. leprae in relation to M. tuberculosis .

Article Snippet: Automated draft reconstructions of M. leprae and My. abscessus were downloaded from BioModels and evaluated against other organism-specific databases, such as BioCyc, (RRID: SCR_002298 ) and Kyoto Encyclopedia of Genes and Genomes (KEGG, RRID: SCR_012773 ) [ , ] .

Techniques: